Original Article Effects of low molecular heparin sodium on HPA and MMP-9 expression in tissues and serum of hypertensive pregnant rats

Yanmin Jiang, Minqin Li, Wei Huang
2016 Int J Clin Exp Pathol   unpublished
Pregnancy induced hypertension (PIH) is one pregnant related disease. Heparanase (HPA) and matrix metalloproteinase-9 (MMP-9) are important degradation enzymes of extracellular matrix. This study established PIH rat model, on which low molecular heparin sodium was applied to analyze its effect on HPA and MMP-9 expression. SPF grade healthy Wistar rats were generated for PIH model using nitro L arginine methyl ester (L-NAME). Low molecular heparin sodium was intraperitoneally injected in
more » ... injected in experimental group, in parallel with normal pregnant rats in control group. Average artery pressure and urea protein levels were measured. Expression of HPA and MMP-9 in serum and placental tissues were measured by ELISA and IHA method, respectively. The correlation between HPA, MMP-9 and PIH was analyzed. Experimental group had lower artery pressure and urea protein levels than model group but higher than blank control group (P<0.05). Serum and placental levels of HPA and MMP-9 were higher in experimental group compared to model group and were lower than blank control group (P<0.05). HPA was positively correlated with MMP-9 expression (P<0.05). Low molecular heparin sodium can decrease average artery pressure and urea protein level in PIH rats, and elevated serum and tissue levels of HPA and MMP-9 in PIH rats, thus exerting certain treatment effects.