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Journal of Pharmaceutical and Biological Sciences
A Novel, stability indicating reversed phase Ultra performance liquid chromatography (UPLC) method has been developed and validated for simultaneous determination of Homatropine Methyl bromide (HM) and Hydrocodone Bitartrate (HB) related compounds in pharmaceutical dosage form. This chromatographic separation was carried out on an Acquity UPLC using reversed phase column (100 x 2.1 mm, 1.7 µm), a simple gradient program for 40 minutes used. Mobile phase A (buffer) consists a mixture of 14.5 mMfatcat:zgvg25jxqzf6lb52udmh6vlula