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The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP
Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiative excited state decay channels are yet poorly understood. In this work we employed resonance Raman and fluorescence spectroscopy to analyse the consequences of multiple amino acid substitutions ondoi:10.14279/depositonce-8640 fatcat:uxlturvifvcoppshmlkyhbxiuq