Antigenic Characterization of Embryo Stage of Setaria cervi

Sunita Saxena, Piyush Dravid, Inayat Sheikh, Nuzhat Kaushal, Deep Kaushal
Filariasis is a serious health hazard affecting millions of people in tropical and subtropical regions of developing countries including India. The specific diagnosis and control measures are essential for effective management of the disease. It has been shown earlier that the excretory-secretory products of filarial parasites contain diagnostically important embryo antigens and it would be important to identify and characterize the embryo stage antigens having potential for serodiagnosis of
more » ... an filariasis. In the present study, efforts were made to analyse the protein and antigenic pattern of embryo stage of Setaria cervi, the bovine filarial parasite. The S. cervi embryos (ScEmb) were isolated by dissecting adult female worms and purified using Percoll gradient. The SDS-Polyacrylamide gel electrophoresis revealed 15-18 protein bands in S. cervi embryo somatic extract in molecular weight range of 6.5-200 kDa. The polyclonal antibodies produced against S. cervi embryo showed significantly high reactivity with ScEmb antigens as well as with antigens from adult and microfilarial stages. The 10-14 protein bands of S. cervi embryo were recognized by anti-ScEmb polyclonal antibodies in immunoblotting. These studies revealed some qualitative and quantitative differences among the antigenic pattern of three stage of S. cervi. The S. cervi embryos exhibited high reactivity with antibodies present in filarial patients' sera thereby suggesting the presence of some antigens common/cross-reactive with human filarial parasite. The protein bands of 60 kDa, 45 kDa, 29 kDa and 27 kDa of S. cervi embryo stage were recognized by filarial patients serum pool and may have diagnostic utility in human lymphatic filariasis  Manuscript