Brychta P., D. Horky, D. Havranova, Z. Janecek, 1. Adler: Model Skin DeJects in Rats Jor the Composite Skin Graft Evaluation. Acta vet. Bmo, 1997,66:23-26. An animal model (rat) was used for the study of the wound healing process. Full-thickness round skin defects, 3 cm in diameter, placed on the dorsal side of the body were made down to muscle fascia. Plastic rings were used to prevent wound contraction and spontaneous epithelialization from the wound edges. In the first step allodermis was

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... pared using trypsin, and applied into the wound bed. Cultured epidermal autografts were prepared in vitro using modified Rheinwald-Green method, and in the second step applied on the taken allodermis. In this way composite skin grafts consisting of allodermis and cultured epidermal autografts were constructed. The semipermeable dressing was changed three-times a week. The healing process was monitored using both the appearance and light microscopic evaluation. In the experimental group of 10 animals uniform uncomplicated healing of these composite skin grafts occurred. After 3 months, the composite skin grafts acquired a form very similar to intact skin missing only the appendages (hair and glands). In the microscopic view only rather flat rete ridges under the dermo-epidermal junction made the difference between the graft and intact skin. Model skin dejects, cultured epidermal autografts, allocorium, composite skin grafts