DNA Chips: from elaboration to application

E. Souteyrand
1999 Analusis  
Figure 2. Principle of DNA chips. On a solid support, a lot of defined areas are drawn. In each area, known oligonucleotides are immobilised (or directly synthesised). Each area corresponds to one type of oligonucleotide. When DNA chips is put in contact with solution containing single strand DNA to be detected, only strands complementary to fixed oligonucleotides can form a stable hybrid in localised area. The detection of different areas where hybridisation occurred allowed determining the
more » ... ious sequences present in solution. 645 ANALUSIS, 1999, 27, N°7 Figure 5. Drops are confined on the surface thanks a previous chemical surface treatment giving hydrophobic or hydrophilic properties on local areas.
doi:10.1051/analusis:1999270639 fatcat:l3tqg2cwbnd6nbu5vjuagybmhe