Machine-assisted cultivation and analysis of biofilms

Silla H. Hansen, Tobias Kabbeck, Carsten P. Radtke, Susanne Krause, Eva Krolitzki, Theo Peschke, Jannis Gasmi, Kersten S. Rabe, Michael Wagner, Harald Horn, Jürgen Hubbuch, Johannes Gescher (+1 others)
2019 Scientific Reports  
Biofilms are the natural form of life of the majority of microorganisms. These multispecies consortia are intensively studied not only for their effects on health and environment but also because they have an enormous potential as tools for biotechnological processes. Further exploration and exploitation of these complex systems will benefit from technical solutions that enable integrated, machine-assisted cultivation and analysis. We here introduce a microfluidic platform, where readily
more » ... le microfluidic chips are connected by automated liquid handling with analysis instrumentation, such as fluorescence detection, microscopy, chromatography and optical coherence tomography. The system is operable under oxic and anoxic conditions, allowing for different gases and nutrients as feeding sources and it offers high spatiotemporal resolution in the analysis of metabolites and biofilm composition. We demonstrate the platform's performance by monitoring the productivity of biofilms as well as the spatial organization of two bacterial species in a co-culture, which is driven by chemical gradients along the microfluidic channel.
doi:10.1038/s41598-019-45414-6 pmid:31222095 pmcid:PMC6586868 fatcat:raajfps6gjalxircru7wagpcj4