Tiger (Panthera tigris), the biggest cat species and the top predator has fallen into endangered category. In the present study, we confirmed the subspecies of tiger with help of DNA based methods. The mitochondrial control region gene (D-loop) sequence was utilized for this purpose. DNA was extracted from tiger blood sample originally obtained from mini zoo of Rohtak, Haryana. The D-loop sequence was amplified and submitted to GenBank with accession number HM362438. Based on D-loop sequence

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... ntity and phylogenetic analysis Panthera tigris tigris (Indian tiger) was differentiated from other Panthera tigris subspecies (Panthera tigris amoyensis, Panthera tigris corbetti, Panthera tigris sumatrae and Panthera tigris altacia) from different parts of the world. The present report also describes an easier, quicker and non hazardous protocol for the isolation of genomic DNA from tiger blood. All copyrights reserved to Nexus® academic publishers