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Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip

2005 Bulletin of the Korean Chemical Society (Print)  

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This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This
more » ... r-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from 10 −18 M to 10 −5 M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.
doi:10.5012/bkcs.2005.26.3.379 fatcat:kmck7kcgn5frxnryqhransmu5a
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"Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip." Bulletin of the Korean Chemical Society (Print) 26.3 (2005) 379-383