KIF1A/UNC-104 transports ATG-9 to regulate neurodevelopment and autophagy at synapses [article]

Andrea K.H. Stavoe, Sarah E. Hill, Daniel A. Colón-Ramos
2016 bioRxiv   pre-print
Autophagy is a cellular degradation process essential for neuronal development and survival. Neurons are highly polarized cells in which autophagosome biogenesis is spatially compartmentalized. The mechanisms and physiological importance of this spatial compartmentalization of autophagy in the neuronal development of living animals are not well understood. Here we determine that, inC. elegansneurons, autophagosomes form near synapses and are required for neurodevelopment. We first determined,
more » ... rough unbiased genetic screens and systematic genetic analyses, that autophagy is required cell-autonomously for presynaptic assembly and for axon outgrowth dynamics in specific neurons. We observe autophagosomes in the axon near synapses, and this localization depends on the synaptic vesicle kinesin, KIF1A/UNC-104. KIF1A/UNC-104 coordinates localized autophagosome formation by regulating the transport of the integral membrane autophagy protein, ATG-9. Our findings indicate that autophagy is spatially regulated in neurons through the transport of ATG-9 by KIF1A/UNC-104 to regulate neurodevelopment.
doi:10.1101/057166 fatcat:wshh7alhszfvzcdrcchlepw6b4