Inhibition of Reactive Oxygen Species/Extracellular Signal-Regulated Kinases Pathway by Pioglitazone Attenuates Advanced Glycation End Products-Induced Proliferation of Vascular Smooth Muscle Cells in Rats

Xiaochen Yuan, Zhengang Zhang, Kaizheng Gong, Pei Zhao, Jianhua Qin, Naifeng Liu
2011 Biological and Pharmaceutical Bulletin  
Advanced glycation end products (AGEs) have been shown to induce the proliferation of vascular smooth muscle cells (VSMCs) and contribute to atherogenesis and diabetes. In the present study, we investigated the effects of pioglitazone, a peroxisome proliferator activated receptor gamma (PPARg g) agonist, on AGE-induced rat VSMC growth and the underlying mechanism. In cultured rat VSMCs, AGE treatment induced VSMC proliferation in time-and dose-dependent manner, while down-regulated the
more » ... ulated the expression of PPARg g. Pretreatment of pioglitazone not only prevented the down-regulation of PPARg g, but inhibited VSMC proliferation and prevented S-phase entry of cell via a G0-G1 block in the presence of AGEs. Western blotting analysis showed that AGE treatment potentiated to activate extracelluar signal-regulated kinases (ERK1/2) by the induction of intracellular reactive oxygen species (ROS) production, since ROS scavenger N-acetyl-L-cysteine pretreatment significantly inhibited AGE-induced ERK1/2 activation. Further, pretreatment with either N-acetyl-L-cysteine or the inhibitor of ERK1/2 activation suppressed AGE-induced proliferation of VSMCs, suggesting a role of ROS/ERK1/2 signaling. Notably, we demonstrated that pretreatment of pioglitazone significantly attenuated AGE-induced ROS and ERK1/2 activation. Collectively, these results suggest that pioglitazone inhibits AGE-induced VSMC proliferation via increasing PPARg g expression and inhibiting ROS/ERK1/2 signaling pathway.
doi:10.1248/bpb.34.618 pmid:21532147 fatcat:p2cmvlcbkfewxo65vqlf7pi6aa