Essential oils of the fruits of three cultivars of Amomum subulatum (Family-Zingiberaceae), such as varlangy, seremna and sawney were isolated. Antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging and FeCl 3 -reducing models and the presence of 1,8-Cineole was quantified using the developed high performance thin layer chromatography (HPTLC) method. In the DPPH scavenging model, the IC 50

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... e of the essential oil of the seremna, varlangy and sawney were found to be 172.3, 216.9 and 274.3 μg/ml, respectively, while in the ABTS scavenging model, the IC 50 value of the seremna, varlangy and sawney were found to be 27.96, 31.34 and 32.49 μg/ml respectively. The antioxidant power of the FeCl 3 reducing model, the absorbance value of the essential oil of the seremna was found comparatively higher than the essential oils of varlangy and sawney. The content of 1,8-cineole was determined by the developed HPTLC densitometric method, and the value of the percentage (mean ± SD, % w/w) content of the essential oils of the seremna, varlangy and sawney were found to be 69.59 ± 1.45%, 48.78 ± 3.21 and 47.84 ± 1.76 respectively. The highest antioxidant value of seremna cultivar may be due to the presence of high content of 1, 8-cineole. The HPTLC method, therefore confirms that monoterpene 1,8-cineole is the main antioxidant compound present in the fruits of A. subulatum.