SUPPLEMENTAL FIGURE LEGENDS Supplemental Figure S1 . LC-MS/MS analysis of Rpl12 derived from wild-type and ∆set11 strains. Rpl12 in wild-type (set11 + ) or ∆set11 mutant cells was isolated by reverse-phase chromatography, digested with GluC, and analyzed using a Finnigan LTQ spectrometer. (A and B) Base peak, ion chromatogram for a 20-min separation of digested Rpl12 peptides for the wild-type (A) and ∆set11 strains (B). Elution time and detected m/z of representative peaks are indicated. A

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... for the peptide fragment spanning residues 1-8 is indicated by "*." MS spectra of the Rpl12 peptide detected at 8.27 min for wild-type and at 8.03 min for the ∆set11 strain are depicted on the right.