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Design and Isolation of Temperature-sensitive Mutants of Gal4 in Yeast and Drosophila
2007
Journal of Molecular Biology
Little is known about mechanisms responsible for the temperature-sensitive (ts) phenotype, or of the transferability of ts mutants of a specific gene between organisms. Using a structure-based approach, nine ts mutants of Gal4 were generated in yeast by mutating four DNA binding residues. Two of these nine yeast ts mutants were cloned into P element vectors under control of the Elav and GMR promoters and transgenic Drosophila lines were generated. These were crossed to UAS reporter lines and
doi:10.1016/j.jmb.2007.05.035
pmid:17553522
fatcat:ib67hngqsndnzoznhadkwmbk3i