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MGMT (O6-methyl-guanine-DNA methyltransferase) is a protein with a specific enzyme activity that is involved in repair of DNA alkylation alterations introduced by classical chemotherapy, such as involving temozolomide (TMZ) use for glioma and Ewing sarcoma tumors. The MGMT methylation biomarker is frequently used for the evaluation of the treatment prognosis in such type of classical anticancer approach. In this article, optimized analytical conditions of a nested MS-PCR method for thefatcat:myxdpj2o3zetdkybkf64xmh3nu