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Three species of the genus Xylaria (Xylariaceae) are presented based on collections from Guilan Province, Iran. Both morphological and molecular characteristics were used in order to identify these species. Xylaria longissima sp. nov. is described and illustrated as a new Xylaria species, and X. cf. striata and X. xylarioides are reported as new records fro Iranian mycobiota. Xylaria longissima and X. xylarioides were collected from wood of unknown dicotyledonous plants and X. cf. striata wasfatcat:npq6ianmenabrf5awwrlsbhloy