Separation of Stem Cells from Follicle, Pulp and Periodontal Ligament of Impacted Third Molar

Maryam Baghizadeh Fini
2020 Advances in Dentistry & Oral Health  
Aim: The goal of this study is the separation and culture of stem cells, which are important in tissue engineering. This basic research was carried out to separate stem cells from different tissues of the third molar impacted teeth (Pulp, Periodontal ligament, Follicle). Methods and Materials: Forty patients with good general health status, in an age range of 15 to 50 years were selected. The teeth were immediately placed in the transfer medium after performing the surgery. After two hours,
more » ... fter two hours, different tissues were detached in the stem cell laboratory, and placed in the main culture medium. The main culture medium consisted of 80% α-Mem, and 20% FCS (fetal calf serum). After three days, if stem cell attachment was observed, the cells were kept in 25 cc flasks (first passage). After 3-14 days, the trypsin enzyme was added to detach the cells. The dishes were checked until the culture medium was full of stem cells. Stem cell factor was used to inhibit the differentiation of stem cells. Upon separation, the cells were stored for a period of two weeks at a temperature of -70 C and subsequently at -196 C in the cell bank. In the next phase, stem cells were diagnosed and confirmed using a monoclonal magnetic antibody, and Real-Time gene expression. Results: Stem cells of follicular tissue proliferated rapidly and attached to the culture medium in a short period of time. Stem cells of pulp proliferated and attached with some delay compared to follicular stem cells. The separation and proliferation of periodontal ligament stem cells were done successfully in just one of the samples. Conclusion: Stem cells can be separated from different dental tissues and used in dental and maxillofacial reconstruction after culturing them.
doi:10.19080/adoh.2020.12.555839 fatcat:jjxbpovupvfv3dphppmvnbnhqu