Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor

Kang-Deuk Seo, Kwang-Pill Lee, Anantha Gopalan, Sang Chung, Yong Lim, Seong-Ho Choi
2007 Sensors  
Enzyme modified electrodes were fabricated with poly(aniline-co-maminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymer film through
more » ... mer film through physical entrapment. In the other method, the amino and -OH groups in the copolymer are utilized to form covalent functionalization with HRP via glutaric dialdehyde as cross-linker/mediator. The conducting copolymer/enzyme modified electrodes prepared by physical entrapment/covalent functionalization of enzyme were tested for electrocatalytic activities towards sensing of H 2 O 2 . Amperometric results indicate that enzyme modified electrode via physical entrapment possesses better electrocatalytic performance over covalent functionalized enzyme electrode.
doi:10.3390/s7050719 fatcat:xx2tuuztd5g4rhra2kwk4pc7cy