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Phosphoproteomic analysis of human mitotic chromosomes identified a chromokinesin KIF4A
2016
Biomedical research
Protein phosphorylation is the prime post-translational modification to drive cell division. Identification of phosphorylated proteins and their related kinases has uncovered molecular networks underlying mitotic processes, including chromosome assembly. Here we aimed to identify phosphoproteins from a mitotic chromosome-enriched lysate biochemically using mass spectrometry. We employed the Polo-box domain (PBD) of Polo-like kinase to tether phosphorylated proteins in the lysate. Resulting
doi:10.2220/biomedres.37.161
pmid:27108885
fatcat:6day4gdutnbwdlm4p76kwbriye