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Background Recent interest in Populus as a source of renewable energy, combined with its numerous available pretreatment methods, has enabled further research on structural modification and hydrolysis. To improve the biodegradation efficiency of biomass, a better understanding of the relationship between its macroscopic structures and enzymatic process is important. Results This study investigated mutant cell wall structures compared with wild type on a molecular level. Furthermore, a noveldoi:10.1186/s13007-020-00691-5 pmid:33292382 fatcat:r43nderqzvhjrmi3hxwklurpka