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Transcription Factor BETA2 Acts Cooperatively with E2A and PDX1 to Activate the Insulin Gene Promoter
2000
Journal of Biological Chemistry
The insulin gene is efficiently expressed only in pancreatic beta cells. Using reverse transcriptase-polymerase chain reaction analysis, we show that insulin mRNA levels are at least 10 5 -fold higher in beta cells than nonbeta cells. To examine the underlying mechanisms, we expressed beta cell transcription factors by transfection of non-beta cells. Separate expression of BETA2, E2A, or PDX1 led to modest (<10-fold) activation of the insulin promoter, whereas co-expression of the three
doi:10.1074/jbc.275.3.2199
pmid:10636926
fatcat:jru2ht7ok5bylpiqbg7v3u5zty