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African Journal of Biotechnology
Pepper leaf curl virus (PepLCV) is the most destructive pathogen of pepper and causes substantial economic losses of chilli production worldwide. Curling and puckering of leaves and stunted growth of the plants are typical symptoms of the viral infection. For a reliable detection of PepLCV, coat protein specific primer pairs were designed and after polymerase chain reaction amplification (PCR), -1 kb amplification product specific to coat protein gene was amplified. Cloning of thisdoi:10.5897/ajb10.1529 fatcat:l7ennjfmbzafjg6imkonlscstq