Soil extracellular enzymes are considered key components in ecosystem carbon and nutrient cycling, and analysing their stoichiometry is an effective way to reveal the resource limitations on soil microbial metabolism. In this study, the soil and litter of Quercus acutissima plots, Pinus thunbergii plots, Quercus acutissima–Pinus thunbergii mixed-plantation plots, herb plots, and shrub plots in the state-owned Dawa Forest Farm in the Yimeng Mountain area were studied. The total carbon (C),

more »

... en (N), and phosphorus (P) contents of litter and the physical and chemical properties of soil were analyzed, along with the activities of four extracellular enzymes related to the soil C, N, and P cycle: β-1,4-glucosidase (BG), β-1,4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and acid phosphatase (AP). The extracellular enzyme stoichiometric model was used to study and compare the metabolic limitations of soil microorganisms in different plots, and the driving factors of microbial metabolic limitations were explored by redundancy and linear regression analyses. The results showed that the values of BG/(NAG + LAP) were all higher than 1, the values of (NAG + LAP)/AP all lower than 1, and the vector angles of the five plots all greater than 45°, which indicated that the soil microorganisms were relatively limited by C and P. Redundancy and linear regression analysis revealed that soil physical properties (e.g., soil moisture) and litter total C make greater contributions to soil extracellular enzymes and stoichiometry than the other investigated soil parameters, whereas soil chemical properties (e.g., soil organic C and available P) predominantly controlled vector properties. Therefore, microbial metabolism limitations are greatly regulated by soil physical and chemical properties and litter total C and N. Compared with the forest plots, the soil microbial C (1.67) and P (61.07°) limitations of herb plots were relatively higher, which means that the soil microbial communities of fore [...]