In this paper, we presented a straightforward strategy to generate 15 combinations with 3 samples, using single-layer microfluidic network. First, we investigated the performance of the single-layer based design by computational simulation (CFD-ACE + ). The simulated output concentrations were extremely close to the expected values within absolute error < 1%. Based on the simulated design, a PDMS device was fabricated by soft-lithography and tested with fluorescent dye (sodium salt). The

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... torial mixing results for 15 combinations showed good performance with absolute error less than 4%. In addition, we have also conceptually presented two liquid handing methods (bottom-up and top-down) for high-throughput screening and assay.