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Transcription factor regulation of RNA polymerase's torque generation capacity
2019
Proceedings of the National Academy of Sciences of the United States of America
During transcription, RNA polymerase (RNAP) supercoils DNA as it translocates. The resulting torsional stress in DNA can accumulate and, in the absence of regulatory mechanisms, becomes a barrier to RNAP elongation, causing RNAP stalling, backtracking, and transcriptional arrest. Here we investigate whether and how a transcription factor may regulate both torque-induced Escherichia coli RNAP stalling and the torque generation capacity of RNAP. Using a unique real-time angular optical trapping
doi:10.1073/pnas.1807031116
fatcat:4iaiqt3znfgjdgt6fle6nwtfoe