Simple Determination of Residual Anticoccidial Drugs(Diclazuril and Nicarbazin) in Chicken Tissues by HPLC

Maki KANDA, Keiko USHIYAMA, Kyoko IGUSA, Mitsunori MURAYAMA, Masakazu HORIE, Masako HIROKADO, Tomoyuki MIYAZAKI
2003 Shokuhin Eiseigaku Zasshi  
A simple and rapid determination of anticoccidial drug residues, diclazuril (DCZ) and nicarbazin (NCZ), in chicken tissues has been developed. DCZ and NCZ were extracted with acetonitrile from chicken liver, muscle, and fat. The extract was rinsed with n-hexane saturated with acetonitrile and then evaporated. The residue was dissolved in 1.4 mL of acetonitrileῌmethanol (1 : 1), then 1.0 mL of n-hexane saturated with acetonitrileῌmethanol (1 : 1) was added, and the mixture was partitioned by the
more » ... addition of 0.6 mL of water. DCZ and NCZ in the aqueous layers were determined by HPLC on an Xterra RP-18 column with acetonitrileῌ0.5 ammonium acetate containing 0.01 mol/L tetra-n-butylammonium hydrogen sulfate (43 : 57) as the mobile phase. The mean recoveries (nΐ5) of DCZ and NCZ spiked in chicken tissues at the maximum residue levels were 92.0ῐ95.6 (CV 2.4ῐ3.0) and 87.3ῐ89.4 (CV 1.7ῐ2.8), respectively. The detection limits of DCZ and NCZ were 0.01 and 0.004 mg/g, respectively.
doi:10.3358/shokueishi.44.110 fatcat:d2ep2qbkfjc53pvc7esw7bwo24