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HotSpot Wizard: a web server for identification of hot spots in protein engineering
2009
Nucleic Acids Research
HotSpot Wizard is a web server for automatic identification of 'hot spots' for engineering of substrate specificity, activity or enantioselectivity of enzymes and for annotation of protein structures. The web server implements the protein engineering protocol, which targets evolutionarily variable amino acid positions located in the active site or lining the access tunnels. The 'hot spots' for mutagenesis are selected through the integration of structural, functional and evolutionary
doi:10.1093/nar/gkp410
pmid:19465397
pmcid:PMC2703904
fatcat:lgix3nuohjg5hicsdnr33vj7aa