Original Article Coexistence and mutual regulation between tyrosine hydroxylase and calcitonin gene-related peptide in facial nucleus of rats regulate the neuronal activities of afferent and efferent neuro fibers

Zhen Zhao, Mike Min, Xiao Gu, Hongliang Zheng, Jianhua Qiu, Jing Zou
2017 Int J Clin Exp Med   unpublished
To explore the coexist meaning of tyrosine hydroxylase (TH) and calcitonin gene-related peptide (CGRP) in facial nucleus, immunohistochemistry and immunofluorescence staining were used to detect whether there is TH expression in facial nucleus and whether there has coexist phenomenon between TH and CGRP. The mRNA levels and protein expression values of TH and CGRP in facial nerve cells were determined before and after transfection with siRNA-TH and shRNA-TH. There were many TH and CGRP
more » ... H and CGRP double-label neuronal somas in ventrolateral sub-nuclear, dorsolateral subnucleus and dorsal intermediate subnucleus of facial nucleus. The nerve fibers packed around and connected with each other like a net. The mRNA levels and protein expression values of TH and CGRP decreased after facial nerve cells transfected with siRNA-TH, while the levels and values increased after transfec-tion with shRNA-TH. In conclusion, TH and CGRP had coexistence phenomenon in facial nucleus. They regulate the neuronal activities of afferent and efferent neuro fibers collectively.
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