MAPK pathway regulated the cardiomyocyte apoptosis in mice with post-infarction heart failure

Q. Zhang, L. Lu, T. Liang, M. Liu, Z. L. Wang, P. Y. Zhang
2017 Bratislava Medical Journal  
BACKGROUND: To explore the role of the MAPK signaling pathway in the cardiomyocyte apoptosis of mice with post-infarction heart failure (HF). METHODS: Mice were divided into sham and myocardial infarction (MI) groups. Before surgery, the MI group was divided into SB203580 and PBS subgroups. A post-infarction HF model was established by ligating the left anterior descending coronary artery. Ventricular dilatation and cardiac function were observed by small animal echocardiography. The growth of
more » ... rimary cardiomyocytes was observed under an inverted phase contrast microscope. The mRNA and protein expressions of endoplasmic reticulum stress (ERS) markers, GRP78 and CHOP, were detected by qRT-PCR and immunofl uorescence assay, respectively. RESULTS: The MI group had enlarged left ventricle and decreased cardiac function. GRP78 and CHOP protein expressions in myocardial tissues, especially those of SB203580 subgroup, signifi cantly increased (p < 0.05). The expressions of p-JNK and cleaved caspase 12 proteins, especially those of SB203580 subgroup, were signifi cantly up-regulated. Cardiomyocytes of MI group were signifi cantly more prone to apoptosis (p < 0.05), with SB203580 subgroup being more obvious. CONCLUSION: MI was accompanied by ERS, probably involving the MAPK signaling pathway. SB203580, a specifi c inhibitor of this pathway, can relieve cardiomyocyte apoptosis and protect the myocardium by suppressing such stress (Tab. 3, Fig. 7 , Ref. 20). Text in PDF
doi:10.4149/bll_2017_065 pmid:28664743 fatcat:gkcptwon5vfypawtkxenyb67he