Role of Dendritic Cells in Chronic HCV Infection and its Correlation with Interferon and Ribavirin Treatment Outcomes

Ahmad Khalaf, A Abdallah, El-Bially, Mohamed Abdeldayem
International Journal of Science and Research   unpublished
Hepatitis C virus (HCV) infects more than 170 million people worldwide and is a major public health risk. This study was conducted to investigate the role of dendritic Cells in Chronic HCV Infection during the course of Interferon and Ribavirin treatment. The study participants will include three groups of patients. First group include 15 non treated patients with chronic HCV infection as a positive control group. The second group include 15 patients with proven chronic HCV infection and
more » ... nfection and received weekly pegylated Interferon and Ribavirin treatment, This group subdivided into two subgroups: Subgroup A non-responder group with (7) patients. Subgroup B responder group with (8) patients. The third group include 30 healthy patients as a negative control group. Mononuclear cells (PBMCs) were separated from peripheral blood and cultured in presence of either R848 or TNF-α for 48 hrs. At the end of culture period, cytokines, IL-10 and IFN-γ were quantified in the culture supernatants by ELISA.