Sepsis imposes a major threat to newborn infants. It is estimated that sepsis causes over half a million neonatal deaths annually, thereby accounting for about 15% of all neonatal deaths worldwide. This study aimed to: Assess the impact of intervention program on nursing performance provided for neonates with sepsis at Intensive Care Units. Setting: This study was conducted at Neonatal Intensive Care Units in Benha University Hospital, Benha Specialized Pediatric Hospital and Benha Teaching

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... ital. Design: A quasi-experimental design was used. Sample: A purposive sample of 100 nurses included in the study working at the previously mentioned settings. Tools: The first tool is a pre-designed questionnaire to assess nurses' knowledge about neonatal sepsis, the second tool was observational checklist to assess nurse's performance for neonates with sepsis. Results: revealed that, the mean age of the studied nurses was 23.9±3.8 years and there was a positive correlation between nurses' knowledge and practices scores post program implementation compared with preprogram implementation. Conclusion: Nurses' performance are significantly improved post program implementation that reflects the importance of continuous and in-service education for nurses working at Neonatal Intensive Care Units. Recommendation:Collaboration and continuing education of the staff in the NICUs are vital to improve the quality of care provided for neonates with sepsis.