Polarization phenotype of peritoneal macrophages in mice with hypertriglyceridemic acute pancreatitis

Yi Yang, Qi-Lin Huang, Chen Luo, Li-Ye Liu, Hong-Yu Sun, Li-Jun Tang
2020 Medical Journal of Chinese People's Liberation Army  
Objective To observe the inflammatory response and peritoneal macrophage polarization phenotype of hypertriglyceridemic acute pancreatitis (HTG-AP) mice, and to record HTG-AP disease progression. Methods Adult male C57/BL6 mice were randomly divided into the control group, HTG group, AP group, and HTG-AP group, with 8 mice in each group. Mice in HTG and HTG-AP groups were intraperitoneally injected with Poloxamer 407 every other day for 28 days to construct the hyperlipidemia animal model. All
more » ... animal model. All animals anesthetized and sampled after caerulein treatment in the AP group and the HTG-AP group 12h later. Blood was collected to detect serum lipid and cytokine levels. HE staining was performed on the pancreatic tissues and pathological scores were obtained. The abdominal cavity was lavage with PBS to collect peritoneal macrophages, which were labeled by F4/80, CD11c, and CD206 and then detected by flow cytometry. Results The HTG-AP model of mice was constructed. Compared with AP group, pancreatic tissue pathology score, serum pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 levels, as well as the percentage of M1 polarization of peritoneal macrophages, were significantly increased (P<0.05), and the level of serum anti-inflammatory cytokine IL-10 and the percentage of M2 polarization of peritoneal macrophages were significantly decreased (P<0.05) in the HTG-AP group. Conclusions Mice in the HTG-AP group showed more severe pancreatic tissue injury and more serious systemic inflammation than those in the AP group, which may be related to the increased ratio of M1 polarization of peritoneal macrophages. DOI: 10.11855/j.issn.0577-7402.2020.08.04
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