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Next Generation Sequencing Enabled Genetics in Hexaploid Wheat
[chapter]
2015
Advances in Wheat Genetics: From Genome to Field
Next Generation Sequencing (NGS) is providing new methodologies to improve and complement traditional genetic approaches. These strategies, collectively termed NGS-enabled genetics, consist of identifying variation in bulks of plants that have been assembled based on a specifi c phenotype of interest. We examined NGS-enabled genetics in hexaploid wheat by using near isogenic lines (NIL) differing across a specifi c disease resistance locus. RNA-Seq of NILs allowed the identifi cation of SNPs
doi:10.1007/978-4-431-55675-6_22
fatcat:zdxv2snefrfcvjskwiwss5eqf4