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Study on the production of bioethanol using palm oil empty fruit bunch (EFB) has been performed using actinomycete Streptosporangium roseum. Positive result of bioethanol production was recorded using Iodoform test followed by confirmation with GC-FID using a polar capillary column (PEG-type, 10m x 0.53, with autosampler) and n-propanol as internal standard. The first and second round distillation has produced azeotrope (85-15% ethanolwater) and the third round has concentrated the ethanol todoi:10.1088/1757-899x/358/1/012032 fatcat:f7hvmfeofnadzeqvarm6fqjgry