Biosorption of Some Heavy Metal Ions Using Bacterial Species Isolated from Agriculture Waste Water Drains in Egypt

Azza Zeid, Wesam Hassanein, Hedayat Salama, Ghada Fahd
2009 Journal of Applied Scienes Research   unpublished
s: The aim of the present investigation is to evaluate the suitability of three agriculture water drains in Sharqia Province region namely Bilbeis, El-Qalyubia and Baher El-Baqar drains, for reuse in irrigation purposes. The chemical analysis of the three water drains revealed that the average concentrations of the heavy metals (Fe , Cd , Zn , Cu , and Pb) were within the normal ranges except 2 + 2 + 2 + 2 + 2 + for cadmium concentration in El-Qalyubia drain and lead concentrations in all
more » ... ations in all drains were over the limited ranges according to Egyptian law 48 (1982). Sixty one bacterial strains naturally inhabiting water drains were isolated; purified and preliminary identified and examined for their ability to uptake Pb and Cd + 2 + 2 from their solutions. Five strains encoded I, II, III, IV and V, were the most frequent among the isolated bacteria in the three drains. Strains I and II and IV were the most resistant isolates for Pb and Cd. + 2 + 2 MICs of Pb for isolates I and II were 40 and 200 ppm and M ICs of Cd for isolates II and IV were + 2 + 2 2.5 and 5.0 ppm, respectively. By examining the effect of increasing concentrations of heavy metals ions on the biological uptake revealed that the maximum uptake of Pb and Cd occurred at sub MIC +2 + 2 concentrations for isolates II and IV (150 ppm&2.5 ppm),respectively. Identification of isolates II and IV were confirmed by Biolog System as Chryseomonas luteola (II) and Pseudomonas mendocina (IV). By studying the influence of environmental conditions, it is obvious that 30°C was optimum temperature for Pb and Cd uptakes by C. luteola and P. mendocina, respectively. Maximum removal capacities of + 2 + 2 the two selected isolates were obtained at pH 7 while pH 8 was suppressive value for both metal ions. Maximum biosorption of Pb by C. luteola was observed after 2 days whereas Cd biosorption by P. + 2 + 2 4 4 mendocina occurred during 3 days of incubation. Upon addition of other cations (MgSO and MnSO) to nutrient media, the uptake of Pb ions by C. luteola decreased from 81.97 mg/g dry wt. (control) to 77.88 + 2 and 74.10 mg/g dry wt., respectively. Similarly, Cd ions removal by P. mendocina decreased from 0.75 + 2 mg/g dry wt. (control) to 0.72 and 0.67 mg/g dry wt., respectively. The plasmid profile of the two selected strains showed that both bacterial isolates harbored plasmids having molecular weights 8 and 9 kbp and concentrations 19.8 and 19.6 µg /µl in case of C. luteola and P. mendocina, respectively.