Internet Archive Scholar

Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity

Christina Kyrousi, Adam C. O'Neill, Agnieska Brazovskaja, Zhisong He, Pavel Kielkowski, Laure Coquand, Rossella Di Giaimo, Pierpaolo D'Andrea, Alexander Belka, Andrea Forero Echeverry, Davide Mei, Matteo Lenge (+12 others)
2021

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (28.5 MB)
https://web.archive.org/web/20220722045123/https://www.research-collection.ethz.ch/bitstream/handle/20.500.11850/514850/s41467-021-26447-w.pdf;jsessionid=EFB21508EB0854CE577CF645013D66DD?sequence=3

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2022; you can also visit the original URL. The file type is application/pdf.

Basal progenitors (BPs), including intermediate progenitors and basal radial glia, are generated from apical radial glia and are enriched in gyrencephalic species like humans, contributing to neuronal expansion. Shortly after generation, BPs delaminate towards the subventricular zone, where they further proliferate before differentiation. Gene expression alterations involved in BP delamination and function in humans are poorly understood. Here, we study the role of LGALS3BP, so far known as a
more » ... ncer biomarker, which is a secreted protein enriched in human neural progenitors (NPCs). We show that individuals with LGALS3BP de novo variants exhibit altered local gyrification, sulcal depth, surface area and thickness in their cortex. Additionally, using cerebral organoids, human fetal tissues and mice, we show that LGALS3BP regulates the position of NPCs. Single-cell RNA-sequencing and proteomics reveal that LGALS3BP-mediated mechanisms involve the extracellular matrix in NPCs' anchoring and migration within the human brain. We propose that its temporal expression influences NPCs' delamination, corticogenesis and gyrification extrinsically.
doi:10.3929/ethz-b-000514850 fatcat:wr7igr4hhnc6tgugepexupbekq
Open Access
Citation

Christina Kyrousi, Adam C. O'Neill, Agnieska Brazovskaja, Zhisong He, Pavel Kielkowski, Laure Coquand, Rossella Di Giaimo, Pierpaolo D'Andrea, Alexander Belka, Andrea Forero Echeverry, Davide Mei, Matteo Lenge, Cristiana Cruceanu, Isabel Y. Buchsbaum, Shahryar Khattak, Guimiot Fabien, Elisabeth Binder, Frances Elmslie, Renzo Guerrini, Alexandre D. Baffet, Stephan A. Sieber, Barbara Treutlein, Stephen P. Robertson, Silvia Cappello. "Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity." (2021)