PROTECTIVE EFFECT OF FOLIC ACID ON OXIDATIVE STRESS INDUCED BY METHIONINE OVERLOAD IN MALE MICE

Tuqa Sabbar Rahi, Wifaq j. albazi, Ali K. Aljarah, Alaa Hussein AL-Safy
2021 Plant Archives  
Methionine is a basic sulfur that contains important amino acid and is necessary for many vital activities necessary in the body, Methionine is considered a preventive agent for many liver damages, But in the case of excessive iron administration, it has a negative effect. One hundred and fifty (150) mice model selected for present study is Albeno pulp-c and their ages between (12-16) a week, One hundred fifty (150) adult mice were randomly divided into 5 groups (thirty each group) and treated
more » ... group) and treated for six weeks. Group I animals were incubated orally tap water, serving as control, Group II animals were incubated orally L-methionine, Group III animals were incubated orally folic acid ,Group IV animals were incubated methionine plus folic acid, and after the end of the experiment, the following criteria were measured GSH, MDA, AST, ALP, ALT, albumin concentration and globulin concentration. methionine overload administration leads to a decrease in concentration of GSH in G2 and an increase in concentration of GSH in G3and G4, and an increase in concentration of MDA in G2 and decrease in concentration of MDA in G3 and G4, and a significant decrease in globulin concentration in G2 compared to G1 and non significant difference between G3,G4 and G1, Also, the albumin concentration in this experiment was found to have a significant increase in G3 compared to G1, and also there was a significant decrease in G2, and there was non significant difference between G4 and G1, a significant increase in activity of AST in G2 compared to G1 and a significant decrease in activity AST in G4 and G3, compared to G1, a significant increase in G2 in the ALT activity compared to G1, and a significant decrease in activity in G3 compared with G1 and there was non significant difference in activity ALT between G1 and G4, Also showed a significant increase in activity ALP in G2 compared to G1 and a significant decrease in activity ALP in G3 and non significant difference in activity ALP in G4 compared to G1, methionine overload may cause oxidative stress in most cells of the body resulting in the release of free radicals in the cells of the tissues. . Protective role of alcoholic extract of black currant (Vitis vinifera L.) on renal function of adult male rats exposed to methionine overload and hydrogen peroxide. Iraqi Journal of Veterinary Sciences, 23(3). Al-Shammry, Z.M.M. and Al-Okaily, B. (2009). Effect of alcoholic extract of black currant (Vitis vinifera L.) on hepatic function and damage induced by hydrogen peroxide and methionine overload in rats. Al-Anbar Journal of Veterinary Sciences, 2(2): 19-34. Au-Yeung, K.; Woo, C.; Sang, F.; Yip, J.; Siow, Y. and O, K. (2004). Hyperhomocysteinemia activates nuclear factor-Kappa Bin endothelial cells via oxidative stress. Circ Res., 94: 28-36. Balkan, J.; Dogru-Abbasoglu, S.; Cevikbas, U.; AYKAÇ-TOKERI, G. and Uysal, M. (2004). Methionine supplementation did not augment oxidative stress, atherosclerotic changes and hepatotoxicity induced by high cholesterol diet in C57BL/6J mice. Journal of nutritional science and vitaminology, 50(4): 258-264.
doi:10.51470/plantarchives.2021.v21.s1.223 fatcat:ide3fhen6vdi7hm2d5agnsqlzq