Decision letter: Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells [peer_review]

Elizabeth A Miller, Vivek Malhotra, Elizabeth A Miller
2020 unpublished
Intracellular transport undergoes remodeling upon cell differentiation, which involves cell type-specific regulators. Bone morphogenetic protein 2-inducible kinase (BMP2K) has been potentially implicated in endocytosis and cell differentiation but its molecular functions remained unknown. We discovered that its longer (L) and shorter (S) splicing variants regulate erythroid differentiation in a manner unexplainable by their involvement in AP-2 adaptor phosphorylation and endocytosis. However,
more » ... cytosis. However, both variants interact with SEC16A and could localize to the juxtanuclear secretory compartment. Variant-specific depletion approach showed that BMP2K isoforms constitute a BMP2K-L/S regulatory system that controls the distribution of SEC16A and SEC24B as well as SEC31A abundance at COPII assemblies. Finally, we found L to promote and S to restrict autophagic degradation and erythroid differentiation. Hence, we propose that BMP2K-L and BMP2K-S differentially regulate abundance and distribution of COPII assemblies as well as autophagy, possibly thereby fine-tuning erythroid differentiation. Cendrowski et al. eLife 2020;9:e58504. DOI: 1 of 26 SHORT REPORT Cendrowski et al. eLife 2020;9:e58504.
doi:10.7554/elife.58504.sa1 fatcat:up5ncy2z5bhwvfl25efq5zhxmu