Synthesis of Different Analogs of Ab(9-16) Peptide Mass spectrometric evidence for heavy metal binding

Monica Jureschi, Brindusa Alina Petre, Laura Ion, Catalina Ionica Ciobanu, Ion Sandu, Gabi Drochioiu
2019 Revista de chimie (Bucuresti)  
Amyloid-β (Aβ) peptides are proteins associated with Alzheimer's disease (AD), because the extracellular Aβ deposits are the main cause of this disorder. The aggregation of Aβ has been shown to depend on the interactions with metal ions, such as copper, zinc, aluminum or iron. The N-terminal sequence of Aβ or Aβ (1-40) peptides, namely Aβ (1-16) peptide fragment, is considered the metal binding site involved in AD neurodegeneration and amyloidogenesis. Therefore, we have investigated different
more » ... eptide sequences to understand the role played by some amino acid residues in metal binding. In this paper, we report the chemical synthesis of Aβ (9-16) peptide and its analogs by Fmoc/tBu strategy and the mass spectrometric evidence for metal ion binding to newly synthesized peptides. MALDI-ToF mass spectrometry proved to be a reliable tool to detect and identify the metal ion complexes of all peptides investigated with copper, iron and zinc ions.
doi:10.37358/rc.19.9.7547 fatcat:bnx6qbxd7zczhe4gwdcjcibbye