DETERMINATION OF THE EFFECTIVENESS OF NANO SILVER ADDITIVE AQUEOUS EXTRACT OF Moringa oleifera L. (Brassicales: Moringaceae) AGAINST ROOT LESION NEMATODE [Pratylenchus thornei Sher & Allen) Chitwood (Nematoda: Pratylenchidae)] UNDER LABORATORY CONDITIONS

Hayriye Didem Saglam Altinkoy, Onur Dura, Ilker Kepenekci
2020 Journal of Global Innovations in Agricultural and Social Sciences  
Pratylenchus thornei (Sher and Allen) Chitwood (Nematoda: Pratylenchidae) is widespread species and caused important damage on cereals, legumes, ornamental plants and vegetables. P. thornei is migratory endoparasitic nematode, which migrate through root tissue and causing extensive root damage. The objective of study was to determine the effectiveness of nano silver additive aqueous extract of Moringa oleifera L. (Brassicales: Moringaceae) against P. thornei under laboratory conditions. P.
more » ... ei was produced on carrot culture. Nematodes were extracted by petri dish methods and keeping them until using at 4°C. P. thornei suspensions were transferred to 24 cell plate which had approximately 50±5 nematodes each cell in 100 µl. Nano silver additive aqueous extract of M. oleifera was prepared and added to each cell at 4 concentrations (168, 84, 42 and 21 ppm) in 1 ml. Distilled water was used as a control. All dishes were kept at 28±2°C. The nematode exposed 24, 48, 72 and 96 hours in four concentration of M. oleifera. P. thornei were considered dead if they did not move when probed with a fine needle. As a result of this experiment, in 48 hours, 168 ppm (90.55±1.74) and 84 ppm (79.79±1.89) showed highly promising mortality. Nano silver additive aqueous extract of M. oleifera was found effective in reducing P. thornei.
doi:10.22194/jgiass/8.886 fatcat:ey7kf3pgpvdthcymrz5kvtsa7i