Fine structure and immunohistochemical evaluation of endometrium in fertile and infertile women with implantation failure
Dicle Medical Journal
Objectives: Being a temporary process, endometrial receptivity is the functional success of endometrial process. In this study, the fertile and recurrent implantation failure (RIF) of individuals with endometrial tissues is analyzed by Transmission Electron Microscopy (TEM) and immunohistochemistry using the E-cadherin. Our goal is not only to make a comparison of endometrial tissues in women with RIF and fertile but also to provide a better understanding of the process and mechanics of the
... echanics of the cellular roof during the implantation of endometrium. Materials and methods: Our research consists of seventeen infertile women who are after In-Vitro fertilization (IVF) / embryo transfer (ET) not occur on pregnancy and ten fertile women were included. Tissue follow-up were performed using routine light microscopy and TEM techniques. Results: In fertile group, markers of endometrial implantation in the surface epithelium, which are pinopodes were found in heavily. In infertile group, there was prominent cilia formation, microvillus and the presence of inadequate pinopod. E-cadherin using immunohistochemistry study was scoring. When immune-reactivity was very pale in the epithelium of the fertile group, infertile groups showed a clear staining. Conclusion: Recent findings; E-cadherin transmits adhesive properties to receptive period of endometrial epithelium as well as, having dual functions. Firstly, the cell surface adhesion is provided, then the separation of epithelial cells and is thought to be suppressed to enable the invasion of the blastocyst. Unlike TIB group, weak staining of epithelium of the fertile group, is important to facilitate implantation of the blastocyst.