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Calibrated feedback illumination for precise conventional fluorescence and PALM imaging applications
[article]
2019
bioRxiv
pre-print
Spatial light modulation using cost efficient digital mirror arrays (DMA) is finding broad applications in fluorescence microscopy due to the reduction of phototoxicity and bleaching and the ability to manipulate proteins in optogenetic experiments. However, the precise calibration of DMAs and their application to single-molecule localization microscopy (SMLM) remained a challenge because of non-linear distortions between the DMA and camera coordinate system caused by optical components. Here
doi:10.1101/718981
fatcat:bssbnjq3mzgirm36kuzuhgdlfm