Rutin Attenuates Methotrexate-Induced Hepatic Oxidative Stress in Rats
Atatürk Üniversitesi Veteriner Bilimleri Dergisi
This study aimed to assess the antioxidant effects of rutin on methotrexate-induced hepatic oxidative stress. Wistar Albino rats (n=24) were separated into 4 groups: control group (C); methotrexate (M, 20 mg/kg, single dose) group; rutin (R, 50 mg/kg/day, for fifteen days) group; and methotrexate + rutin [MR, methotrexate (20 mg/kg, single dose) + rutin (50 mg/kg/day, for fifteen days)] group. Rats were sacrificed on the sixteenth day and liver tissues were removed. The liver
... dehydrogenase (G6PD), glutathione peroxidase (GPx), malondialdehyde (MDA), nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), and vitamin C were determined colorimetrically. Malondialdehyde increased and vitamin C decreased in the M group compared to the others (P<0.001). Total glutathione reduced in both M and MR groups compared to C group (P<0.05). An important decrease in M group NADPH and GPx compared to C group (P<0.001) was observed. Nicotinamide adenine dinucleotide phosphate of R group were determined to be higher than M group (P<0.001). In this study, it might demonstrate that methotrexate caused oxidative stress in the liver and rutin had antioxidant effects on methotrexate-induced hepatic oxidative stress. Consequently, it can be suggested that rutin may be useful in attenuating the side effects of methotrexate on the liver.