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To extract essential oil from Melaleuca bracteata leaves without thermal degradation, ultrasound-assisted extraction (UAE) was developed and optimized using a response surface method (RSM) based on the Box-Behnken design (BBD). Under the optimized extraction conditions, a higher essential oil yield of 4.55% was achieved in comparison to that of 1.02% via the conventional hydrodistillation extraction method, which suggested that UAE could be used as an alternative and efficient extraction methoddoi:10.15376/biores.13.4.8488-8504 fatcat:2pulvqomxfh4bes64e5qdwlymm