Hybrid rice production can ensure to get more yields per unit land. The research work was carried out to evaluate the effect of number of seedling hill-1 and spacing on the growth and yield of hybrid rice line Bio-453. The experiment was laid out in a split-plot design with 3 replications at the field of Agronomy and Haor Agriculture Department of Sylhet Agricultural University, under the AEZ 20 in Kharif-II (Aman) season of 2013. Number of seedling hill-1 was assigned in the main plot and

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... spacing in the sub-plot. Two levels of seedling hill-1 viz. NS1 (1 seedling hill-1) and NS2 (2 seedlings hill-1) and five levels of plant spacing viz. Sp1 (15 cm X 15 cm), Sp2 (15 cm X 20 cm) and Sp3 (20 cm X 20 cm), Sp4 (20 cm X 25 cm) and Sp5 (20 cm X 30 cm) were the treatments. Number of seedling hill-1 and plant spacing showed significant effect in yield and yield contributing parameters except days to maturity, plant height, 1000-grain weight, total tillers hill-1, number of effective tillers hill-1, harvest index and grains panicle-1. Maximum grain yield (9.43 t ha-1), straw yield (16.27 t ha-1) and biological yield (25.70 t ha-1) were obtained from 1 seedling hill-1 (NS1) with the closest spacing 15 cm X 15 cm Therefore, one seedling hill-1 with 15 cm distance for plant and row to row distance could ensure maximum yield of hybrid rice line Bio-453. Bangladesh Agron. J. 2019, 22(1): 27-37