Intrinsic Signal Amplification by Type-III CRISPR-Cas Systems Provides a Sequence-Specific Viral Diagnostic [article]

Andrew Santiago-Frangos, Artem A. Nemudryi, Anna Nemudraia, Laina N. Hall, Pushya Krishna, Tanner Wiegand, Royce A. Wilkinson, Deann T. Snyder, Jodi F. Hedges, Mark A. Jutila, Matthew P. Taylor, Blake Wiedenheft
2020 medRxiv   pre-print
To combat viral pandemics, there is an urgent need for inexpensive new technologies that enable fast, reliable, and scalable detection of viruses. Here we repurposed the type III CRISPR-Cas system for sensitive and sequence specific detection of SARS-CoV-2 in an assay that can be performed in one hour or less. RNA recognition by type III systems triggers Cas10-mediated polymerase activity, which simultaneously generates pyrophosphates, protons and cyclic oligonucleotides. We show that amplified
more » ... products of the Cas10-polymerase are detectable using colorimetric or fluorometric readouts.
doi:10.1101/2020.10.14.20212670 fatcat:pzeveqrpcjhfnjy7ijcvdkisyu