Human Proteome Project Mass Spectrometry Data Interpretation Guidelines 3.0

Eric W. Deutsch, Lydie Lane, Christopher M Overall, Nuno Bandeira, Mark S. Baker, Charles Pineau, Robert L. Moritz, Fernando Corrales, Sandra Orchard, Jennifer E. Van Eyk, Young-Ki Paik, Susan T. Weintraub (+2 others)
2019 Journal of Proteome Research  
The Human Proteome Organization's (HUPO) Human Proteome Project (HPP) developed Mass Spectrometry (MS) Data Interpretation Guidelines that have been applied since 2016. These guidelines have helped ensure that the emerging draft of the complete human proteome is highly accurate and with low numbers of false-positive protein identifications. Here, we describe an update to these guidelines based on consensus-reaching discussions with the wider HPP community over the past year. The revised 3.0
more » ... elines address several major and minor identified gaps. We have added guidelines for emerging data independent acquisition (DIA) MS workflows and for use of the new Universal Spectrum Identifier (USI) system being developed by
doi:10.1021/acs.jproteome.9b00542 pmid:31599596 pmcid:PMC6986310 fatcat:qiplkvgpgfdldco2tw5d34dpea