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Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe
Peroxynitrite (ONOO-) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO- in cell biology, improved methods for the selective detection and real-time analysis of ONOO- are needed. We present a water-soluble, luminescent europium(iii) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probedoi:10.34657/5827 fatcat:ons3m2d3tfggdeberlv4q7vkpe