Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe [article]

Colum Breen, Robert Pal, Mark R. J. Elsegood, Simon J. Teat, Felipe Iza, Kristian Wende, Benjamin R. Buckley, Stephen Butler, University, My
Peroxynitrite (ONOO-) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO- in cell biology, improved methods for the selective detection and real-time analysis of ONOO- are needed. We present a water-soluble, luminescent europium(iii) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe
more » ... to measure ONOO- in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(iii) probe, we monitored the production of ONOO- in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment. This journal is © The Royal Society of Chemistry.
doi:10.34657/5827 fatcat:ons3m2d3tfggdeberlv4q7vkpe