Abstracts CORRELATION BETWEEN COPY NUMBER OF MITOCHONDRIAL DNA AND CLINICOPATHOLOGIC PARAMETERS OF HEPATOCELLULAR CARCINOMA SERUM TUMOR MARKERS IN SKELETAL METASTASIS

Suguru Yamada, Satoshi Tsukushi
2006 unpublished
Aims: In the current study, we investigated possible correlations of the mtDNA copy number in hepatocellular carcinoma (HCC) with the pathological fi ndings and prognosis. Methods: We studied 31 HCC specimens using quantitative real-time polymerase chain reaction analysis, and the correlation between the mtDNA copy number and the clinicopathologic parameters and mutations in the D-loop region of the mitochondrial genome. Results: The mtDNA copy number was reduced in HCCs compared with the
more » ... ared with the corresponding noncancerous liver tissues (p = 0.002), and signifi cantly correlated with tumor size (p = 0.014) and cirrhosis (p = 0.048). Patients with a low mtDNA copy number tended to show shorter 5-year survival rates than patients with a high mtDNA copy number when assessed by Kaplan-Meier curves, but not a signifi cant (overall survival rate, 63% vs. 83%; p = 0.19). The copy number of HCC with mtDNA D-loop mutation or deletion was lower, but not signifi cantly so (p = 0.656, p = 0.590, respectively). Conclusions: Our results indicated that a reduced copy number of mtDNA is correlated with HCC and associated with malignant potential. Background: There have been no well-documented reports detailing the relationship between skeletal metastasis and tumor markers in a large series of patients. The purpose of our study was to assess the relationship between the clinical features of skeletal metastasis and serum tumor markers and to determine whether tumor markers are a useful modality in the differential diagnosis of skeletal metastasis. Methods: We retrospectively reviewed consecutive 458 patients with skeletal metastasis and divided the patients into two groups according to six clinical presenting factors. We assessed whether these groups infl uenced the level of the tumor markers in univariate and multivariate analysis. Results: Patients with skeletal metastasis of carcinoma had a higher level of markers CEA (P < 0.0001) and CA19-9 (P = 0.0008) than patients with primary bone tumors and hematological malignancies. Univariate analysis of clinical variables revealed that metastasis on axial skeleton, multiple skeletal metastases and visceral metastasis were associated with the serum CEA and CA19-9 levels. By multivariate analysis, metastasis on axial skeleton, multiple skeletal metastases
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