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Positive detection of mycoplasma contamination by the whole-mount preparation of cell cultures for transmission electron microscopy
1978
Journal of Bacteriology
Preserved Fulltext
Low-level mycoplasma contamination of cell cultures is difficult to recognize with presently available techniques. This report describes the adaptation of the whole-mount technique, usually used for scanning microscopy, for transmission electron microscopy. The differentiation between microvilli and the equal-sized filamentous mycoplasma is based on the differential density obtained by the use of the method described. This method allows positive identification of mycoplasma and reduces the
doi:10.1128/jb.133.3.1452-1456.1978
fatcat:xnnncl6usveghpkvpvm6u6cxfa